All antibodies produced by ABCD Antibodies are recombinant.
Recombinant antibodies are antibodies with known sequences that are produced in vitro. These chemically-defined antibodies offer:
- controlled production
- high batch-to-batch consistency
- long-term supply (as the sequence information cannot be lost)
- the possibility of endless antibody engineering
...and don't require the use of animals for their production. Recombinant antibodies are in line with the 3Rs principle.
The problem with traditional antibodies:
Antibodies are among the most widely-used tools in biological sciences. However, they are also one of the major cause of the reproducibility crisis plaguing bio-medical research. Reproducibility involves the ability to repeat experiments with reagents identical to those used by previous researchers.
In the case of antibodies, it is almost impossible to guarantee the use of identical reagents. Polyclonal antibodies are a variable mixture of several undefined antibodies produced by immunization of an animal. Monoclonal antibodies are produced ex vivo from a single clonal cell line (called hybridoma), isolated from an immunized animal. However, hybridomas are not always a homogeneous cell population, and in one-third of cases additional antibody genes might be present. This is an enormous problem in terms of specificity, affinity and reproducibility. Finally, when hybridomas cells producing monoclonal antibodies are lost, the corresponding monoclonal antibody is irretrievabally lost. The only way to guarantee the identity of an antibody reagent is by knowing its aminoacid sequence.
At ABCD Antibodies, we are convinced that recombinant antibodies are superior products and that the entire scientific community would benefit using these reagents. Better antibodies for better science.
Discover what the production format is for our recombinant antibodies and how we are able to adapt the production to your needs.
Find out who we are and why we want to lead this technological revolution.